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. 2013 Apr 23;11(4):e1001542. doi: 10.1371/journal.pbio.1001542

Figure 2. Decreased GH storage and secretion in PICK1-deficient mice.

Figure 2

(A) The livers of PICK-1-deficient mice had a significantly lower weight than those of WT mice (**p<0.01). (B) PICK1-deficient mice exhibited a decreased level of plasma IGF-1 (*p<0.05). (C) Decreased relative expression level of IGF-1 mRNA in the liver of PICK1-deficient mice versus WT mice (**p = 0.0045). Measurements in (A), (B), and (C) were performed in samples from 35–38-wk-old WT (+/+) or PICK1-deficient mice (−/−) after overnight fast. Data are means ± SE (n = 10–14). (D) PICK1-deficient mice display reduced femoral length. Measurements were done on 39–41-wk-old WT (+/+) and PICK1-deficient mice (−/−). Data are means ± SE (n = 4–5). *p<0.05, compared to WT. (E) PICK1-deficient mice display decreased ghrelin-induced GH secretion. Basal plasma GH level and the level 5 min after ghrelin administration were determined in anesthetized WT (+/+) and PICK1-deficient mice (−/−) at the age of 7–9 wk. White bars, WT (+/+); black bars, PICK1-deficient mice (−/−). Data are means ± SE (n = 7–9). *p<0.05, compared to WT. (F) PICK1-deficient mice display decreased GH content in the pituitary. Pituitaries were taken from 34-wk-old WT (+/+) or PICK1-deficient mice (−/−). Data are means ± SE (n = 4). **p<0.01. (G) Relative expression levels of GH and GHR mRNA in pituitaries, determined by RT-PCR analysis (35–38-wk-old mice). White bars, PICK1 +/+: black bars, PICK1 −/−. Data are means ± SE (n = 7–8). *p<0.05.