Table 2.
Characteristics of polymorphic microsatellite loci isolated from M. acuminata Calcutta 4 and Cavendish Grande Naine unigene sets
| Locus number | Unigene ID | SSR repeat motif | SSR locus length (bp) | Allelic Amplitude (bp) | Number of alleles | He | Ho | PIC Value |
|---|---|---|---|---|---|---|---|---|
| locus821 |
musa_c4_small_c22955 |
CGG |
14 |
170-180 |
3 |
0.61 |
0.52 |
0.53 |
| locus945 |
musa_c4_small_c25361 |
TGACA |
17 |
325-330 |
3 |
0.62 |
0.57 |
0.55 |
| locus1284 |
musa_c4_small_c7084 |
AG |
11 |
270-330 |
6 |
0.77 |
0.65 |
0.74 |
| locus1412 |
musa_c4_small_c8798 |
TGC |
15 |
175-190 |
3 |
0.39 |
0.14 |
0.34 |
| locus1654 |
musa_c4_small_rep_c12642 |
CCT |
16 |
170-200 |
5 |
0.70 |
0.71 |
0.66 |
| locus2108 |
musa_c4_small_rep_c2099 |
GA |
14 |
190-220 |
7 |
0.81 |
0.92 |
0.79 |
| locus2061 |
musa_c4_small_rep_c1992 |
AG |
19 |
190-200 |
5 |
0.70 |
0.52 |
0.66 |
| locus2108 |
musa_c4_small_rep_c2099 |
GA |
14 |
205-230 |
8 |
0.84 |
0.91 |
0.82 |
| locus2112 |
musa_c4_small_rep_c21184 |
GTC |
18 |
200-205 |
3 |
0.52 |
0.65 |
0.46 |
| locus2306 |
musa_c4_small_rep_c2740 |
TG |
13 |
170-185 |
4 |
0.74 |
0.38 |
0.69 |
| locus2984 |
musa_c4_clusters_pos_c1484_1 |
TCCT |
13 |
115-130 |
7 |
0.80 |
0.45 |
0.77 |
| locus371 |
musa_cav_c18572 |
AT |
12 |
350-360 |
3 |
0.56 |
0.54 |
0.48 |
| locus2028 |
musa_cav_rep_c22610 |
CTT |
17 |
280-290 |
4 |
0.70 |
0.75 |
0.65 |
| locus2646 | musa_cav_rep_c5186 | TTC | 18 | 200-220 | 5 | 0.73 | 0.30 | 0.68 |
Polymorphism was evaluated across 21 M. acuminata accessions. HE, expected heterozygosity under Hardy-Weinberg expectations; HO, observed heterozygosity; PIC, Polymorphism Information Content.