Table 3.
Primer sequences for qRT-PCR analysis of expression of SSR marker–derived genes associated with defense responses and BAC clone-derived LRR receptor-like serine/threonine-protein kinases
| Gene / contig abbreviation | KOG Gene description / BAC annotation | Primer Sequence Forward/Reverse | Amplicon Size (bp) | PCR efficiency (%) | Efficiency SD (+/−) |
|---|---|---|---|---|---|
| THAU_musa_c4_small_rep_c3564 |
Pathogenesis related proteins, group 5, Thaumatin |
CCGGTGGGACTAATTACAGG/ CAATTCGGATGTCAATGCAG |
165 |
99 |
0,011 |
| CHIT_musa_c4_small_c3092 |
Chitinase |
CACCATCTCCTGCAAGCATA/ GCAGTCATTCCTCGTTGTCA |
123 |
96 |
0,008 |
| M09A11_454_Ma4140M09A11 |
Putative LRR receptor-like serine/threonine-protein kinase |
CCAGCAACCACAACCCTAGT/ GATCTTTCAGGCCTCGTTTG |
176 |
99 |
0,009 |
| B03A51_454_Mac054B03A51 |
Putative LRR receptor-like serine/threonine-protein kinase |
ATTCCATGGCTACGGACAAT/ TTCAGGCCTCGTTTGAACTC |
192 |
107 |
0,005 |
| B03A11_454_Mac054B03A11 |
Putative LRR receptor-like serine/threonine-protein kinase |
CAGCAACCATAACCCCATTC/ AGGATCTTTCAGGCCTCGTT |
168 |
105 |
0,011 |
| EF | Elongation Factor | AACCCCCAAATATTCCAAGG/ AGATTGGCACGAAAGGAATC | 107 | 102 | 0,013 |
Differential gene expression in M. acuminata Calcutta 4 inoculated with M. musicola was analyzed in relation to non-inoculated controls.