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. 2013 Feb 18;8:27. doi: 10.1186/1746-1596-8-27

Table 3.

Intra- and inter-observer agreement based on slides treated with different antigen retrieval buffers

Antigen retrieval buffer
Inter-observer agreement
Fleiss’ Kappa
Intra-observer agreement
Cohen’s Kappa
  Value 95% CI Value 95% CI
EDTA (pH 8.0)
0.912
(0.862, 0.912)
0.955
(0.918, 0.992)
Na Citrate (pH 6.0)
0.753
(0.677, 0.829)
0.853
(0.790, 0.916)
EDTA (pH 9.0)
0.643
(0.558, 0.728)
0.801
(0.730, 0.872)
P < 0.05   < 0.05