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RT-PCR and Western blot analysis in H-460 cells. (A) Effect of combination of P276-00 and doxorubicin or either of the drugs alone on the protein levels 1. Control, 2. P276-00, 3. doxorubicin, 4. doxorubicin> P276-00. (B) mRNA analysis of cyclin B1, Cdk-1, cyclin D1, Cdk-4 and Cox-2. Samples were obtained from H-460 cells treated with 100 nM of doxorubicin and/or 1200 nM P276-00 at various time points as indicated in the figure.
