Figure 1.

Overview of the computational workflow of STOP. The left side illustrates the backend of the STOP software, i.e. the automatic annotation pipelin: (1) The genome and proteome of all included species is retrieved from UniProt and Entrez Gene respectively and subsequently (2) descriptions for all genes/proteins are collected from UniProt and Entrez Gene and finally (3) submitted to the NCBO annotator web service. The information is stored in a MySQL database and can be accessed by the frontend, which is displayed here on the right side. The real-time analysis pipeline requires a list of genes as input and calculates for each term of the 200+ ontologies whether it is enriched in the given gene list. The results are subsequently presented as a tag cloud or in list form