Table 1.
Primers and amplification conditions used in this study
| Primer | Sequences (5’-3’) | Target size (bp) | Annealing temp. (°C) | Reference |
|---|---|---|---|---|
|
toxR-F |
GACCAGGGCTTTGAGGTGGACGAC |
217 |
65 |
[32] |
|
toxR-R |
AGGATACGGCACTTGAGTAAGACTC |
|
|
|
| VFLU-F |
ATAAAGTGAAGAGATTCGTACC |
278 |
60 |
[33] |
| VFLU-R |
GTATTCCTGAATGGAATACAC |
|
|
|
|
Int IV-F |
AACACCGCTTGCACCTCTAT |
525 |
53 |
[34] |
|
Int IV-R |
TGTATGCGCTTGAGAGTCC |
|
|
|
|
stn-F |
GGTGCAACATAATAAACAGTCAACAA |
375 |
53 |
[44] |
|
stn-R |
TAGTGGTATGCGTTGCCAGC |
|
|
|
|
sulII-F |
AGGGGGCAGATGTGATCGAC |
606 |
55 |
[35] |
|
sulII-B |
TGTGCGGATGAAGTCAGCTCC |
|
|
|
|
vfh-F |
GCGCGTCAGTGGTGGTGAAG |
800 |
61 |
This study |
|
vfh-R |
TCGGTCGAACCGCTCTCGCTT |
|
|
|
|
hupO-F |
ATTACGCACAACGAGTCGAAC |
600 |
56 |
This study |
|
hupO-R |
ATTGAGATGGT AAACAGCGCC |
|
|
|
|
vfpA -F |
TACAACGTCAAGTTAAAGGC |
1790 |
55 |
This study |
|
vfpA -R |
GTAGGCGCTGTAGCCTTTCA |
|
|
|
|
arc-F |
AGTTTATGCGTCTGGCTTG |
3427 |
56 |
This study |
|
arc-R |
ATGAGTAAGTTATACGTAGG |
|
|
|
|
arc-rev |
GCTTCGGCCCACATAATAA (paired with arc-F) |
2170 |
56 |
This study |
| arc-ck-up | TTACCACCTAATGCGACGA (paired with arc-R) | 1235 | 56 | This study |