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. 2013 Apr 4;13:179. doi: 10.1186/1471-2407-13-179

Table 1.

BAL fluid cytokine levels in mice 16 weeks post-XRT

  Cytokine values (pg/ml) (mean ± SEM)
 
 FGF-basic
 IL-5
 IL-6
 IL-12 (p40/p70)
 KC
 MCP-1
 VEGF
No XRT (13.5 Gy)
 0% FLC
 ND1
 4.6 ± 0.6
 3.7 ± 0.2
 3.2 ± 1.2
 ND1
 3.3 ± 0.9
 10.9 ± 1.5
 10 % FLC
 ND1
 2.3 ± 0.6a
 2.6 ± 0.8
 0.2 ± 0.1
 ND1
 3.9 ± 1.0
 8.5 ± 1.8
 20% FLC
 ND1
 2.1 ± 0.3a
 2.4 ± 0.6a
 ND1
 ND1
 2.7 ± 0.8
 5.7 ± 0.5a
XRT (+24 Hours)
 0% FLC + 13.5 Gy
 52.5 ± 5.5b
 15.3 ± 5.3
 129.0 ± 86.6
 114.5 ± 24.5b
 261.8 ± 52.6
 77.9 ± 39.7
 47.2 ± 5.0b
 10% FLC + 13.5 Gy
 55.3 ± 5.4
 5.2 ± 0.9
 ND1
 73.4 ± 25.9
 18.1 ± 4.4
 27.2 ± 0.7
 52.1 ± 9.3
 20% FLC + 13.5 Gy
 36.0 ± 6.4
 10.7 ± 4.5
 49.1 ± 22.3
 91.1 ± 21.4
 ND1
 29.1 ± 0.9
 38.4 ± 3.3
XRT (+48 Hours)
 0% FLC + 13.5 Gy
 52.5 ± 5.5
 15.3 ± 5.3
 129.0 ± 86.6
 114.5 ± 24.5
 261.8 ± 52.6
 77.9 ± 39.7
 47.2 ± 5.0
 10% FLC + 13.5 Gy
 61.7 ± 4.8
 2.1 ± 0.3c
 38.4 ± 12.8
 6.7 ± 2.3c
 ND1
 26.8 ± 1.5
 7.2 ± 3.0b
 20% FLC + 13.5 Gy
 37.5 ± 8.6
 7.3 ± 3.6
 132.3 ± 75.0
 83.7 ± 34.6
 ND1
 28.2 ± 1.7
 39.7 ± 11.8
XRT (+72 Hours)
 0% FLC + 13.5 Gy
 52.5 ± 5.5
 15.3 ± 5.3
 129.0 ± 86.6
 114.5 ± 24.5
 261.8 ± 52.6
 77.9 ± 39.7
 47.2 ± 5.0
 10% FLC + 13.5 Gy
 35.6 ± 11.2
 13.7 ± 7.6
 369.7 ± 241.7
 88.1 ± 32.5
 425.7 ± 141.9
 82.5 ± 49.9
 33.0 ± 10.5
 20% FLC + 13.5 Gy 34.8 ± 8.8 2.3 ± 0.8c ND1 22.7 ± 11.8c ND1 23.1 ± 0.7 30.2 ± 9.5

1ND = Not Detectable in Assay.

a = p≤0.05 for non-irradiated 0% FLC vs. non-irradiated 10% FLC and 20% FLC.

b = p<0.01 for non-irradiated 0% FLC vs. irradiated 0% FLC.

c = p<0.05 for irradiated 0% FLC vs. irradiated 10% FLC and 20% FLC.

Mice were pre-fed 0% FLC for 72 hours prior to single fraction thoracic X-ray radiation therapy (13.5 Gy). Following XRT exposure, mouse cohorts (n=15) were fed 10% FLC or 20% FLC diets initiated 24, 48, or 72 hours post-XRT. Control-fed mouse cohorts remained on 0% FLC diet throughout the course of the study. Mice were sacrificed at 16 weeks post-XRT and bronchoalveolar lavage (BAL) fluid was collected. BAL cytokine analysis was performed and data is represented as mean ± SEM. a= p≤0.05 for non-irradiated 0% FLC vs. non-irradiated 10% FLC and 20% FLC, b= p<0.01 for non-irradiated 0% FLC vs. irradiated 0% FLC, and c= p<0.05 for irradiated 0% FLC vs. irradiated 10% FLC and 20% FLC.