Table 1.
Primary antibodies and dilutions used for the immunohistochemical analyses and results
| Antibody to | Manufacturer | Dilution | Results |
|---|---|---|---|
| ER |
Ventana |
ready-to-use |
Negative |
| PgR |
Ventana |
ready-to-use |
Negative |
| HER2 |
Dako |
1:1000 |
Negative |
| S100 protein |
Dako |
1:2000 |
Positive |
| EMA |
Ventana |
ready-to-use |
Positive |
| GCDFP15 |
Signet |
1:20 |
Negative |
| P53 |
Novocastra |
1:200 |
Positive |
| AACT |
Dako |
1:1000 |
Positive |
| Actin |
Dako |
1:400 |
Negative |
| Maspin |
Pharmingen |
1:500 |
Negative |
| Calponin | BioGenex | 1:400 | Negative |
ER, estrogen receptor; PgR, progesterone receptor; HER2, human epidermal growth factor receptor 2; EMA, epithelial membrane antigen; GCDFP15, gross cystic disease fluid protein-15; AACT, alpha-1-antichymotrypsin.