Figure 4. Potential factors involved in snoMEN machinery.

(A) U2OS^{GFP–SMN1-PR} and U2OS^GFP–SMN1 cells were transfected with either Scrambled siRNA, Ago1 siRNA, Ago2 siRNA, Upf1 siRNA, or fibrillarin siRNA. An equivalent amount of each extract was loaded in each lane and the proteins were separated by SDS PAGE, electroblotted onto membrane and probed with anti-SMN1, anti-Ago1, anti-Ago2, anti-Upf1, anti-fibrillarin and with anti-tubulin as a loading control. (B) The graphs show average SMN signal intensity and standard deviation for three independent experiments using the same procedure as in A. SMN signal ratio was normalised to the tubulin signal.