Figure 2.

Residues replaced in Gα₁₂ cassette mutants. For each mutant, designated in italics (A-Z, AA-ZZ, AAA-KKK), the native amino acid sextet replaced by the sequence Asn-Ala-Ala-Ile-Arg-Ser is shown. An arrow between Pro¹³⁹ and Val¹⁴⁰ indicates the site of myc tag insertion. Mutant W was not produced. The dashed box indicates the native Gln²²⁹ mutated to Leu to render Gα₁₂ constitutively active. The native residues replaced in mutant KKK are Lys-Asp-Ile-Met-Leu-Gln and thus partially overlap with mutant JJJ. All cassette mutants contain the activating Q²²⁹L mutation, except mutant LL due to its cassette substitution.