Table 2.
Gα12 cassette mutants corresponding to rgRGS contact points within Gα13
| Gα13 residues in contact with p115-RH | myc-Gα12QL NAAIRS mutant |
|---|---|
| Val98 |
Q |
| Asp101, Ala102 |
R |
| Lys105, Leu106 |
S |
| Thr127, Arg128 |
V |
| Phe168 |
BB |
| Arg200, Pro202, Lys204 |
HH |
| Gln226 |
LL |
| Arg230, Lys231, Phe234 |
MM |
| Met257 |
QQ |
| Arg260 |
RR |
| Asn270 |
SS |
| Ile271, Glu273, Thr274, Ile275 |
TT |
| Asn278, Arg279, Val280 |
UU |
| Arg335 | DDD |
Gα13-native residues previously identified as providing contact points with the RH domain of p115RhoGEF [20,21] are indicated in the left column. Cassette mutants (“NAAIRS” substitution) in which the homologous residue(s) within Gα12 have been altered are indicated in the right column. See Figure 2 for Gα12 mutant designations.