Figure 5. Functional effects of FecX^Gr and FecX^O mutations on the BMP15 activity.

In vitro reporter luciferase assay from COV434 granulosa cells transiently transfected with empty vector +/− 100 ng of recombinant human BMP15 (Control +/− rhBMP15) or wild-type human BMP15 expressing vector (WT) or the 2 different BMP15 variant vectors (BMP15^T317I (FecX^Gr); BMP15^N337H (FecX^O)) obtained by directed-mutagenesis. Results are expressed as Means±SD of the relative light unit (RLU) from 3 independent experiments in triplicate for each condition. Pairwise statistical comparisons using a one-way ANOVA test between means were performed and results of statistic test are symbolized by stars: * = p<5E⁻⁰²; ** = p<1E⁻⁰² and *** = p<1E⁻⁰³.