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. 2013 Apr 25;9(4):e1003474. doi: 10.1371/journal.pgen.1003474

Figure 2. T-STAR is not required for male fertility in the mouse.

Figure 2

(A) Histology of testis from wild type and Khdrbs3−/− animals. Paraffin-embedded sections were probed for T-STAR using a T-STAR specific antibody or Sam68 using a Sam68 specific antibody and counterstained using haematoxylin. The scale bar is equivalent to 20 µm. (B) Litter sizes obtained for HET (n = 40 litters) and Khdrbs3−/− (n = 23 litters) mice. Slightly smaller than average litter sizes were observed for the KO mice. (C) Testis-body weight ratios were not significantly different between WT (Khdrbs3+/+), HET and KO mice indicating no significant defect in adult testis development in the absence of T-STAR protein on a C57BL6 background or a mixed C57Bl6-129 background. (D) Sperm counts of wild type (n = 4); HET (n = 10) and KO (n = 9) mice indicate no reduction in sperm count associated with the knockout allele. (E) Mendelian ratios of litters born from breeding HET mice correspond to the expected 1∶2∶1 ratio, and so indicate no lethality associated with the KO allele (n = 40 litters).