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. 2013 Apr 25;9(4):e1003474. doi: 10.1371/journal.pgen.1003474

Figure 9. T-STAR mediates splicing repression of Nrxn2 AS4 via a downstream response element.

Figure 9

(A) Nucleotide sequence immediately downstream of the Nrxn2 alternative exon (exon sequence shaded grey, intron sequence unshaded) of wild type gene and after mutagenesis to remove U((U/A)AA motifs. (B) Capillary gel electrophoretogram to show splicing response of mutated Nrxn2 minigene after co-transfection with the indicated expression constructs. (C) Percentage Splicing Exclusion levels of the mutated Nrxn2 minigene from 3 independent transfection experiments. The p values were calculated using unpaired t tests, and estimate statistical significance between splicing exclusion of the mutated Nrxn2 minigene on co-expression of GFP(lane 1: shown as green bar) and each of the individual fusion proteins (lanes 2,4–7: shown as black bars). (D) Coomassie stained gel showing purified Sam68-GST and T-STAR-GST fusion proteins used for gelshift experiments. (E) EMSA experiment using RNA probe containing the Nrxn2 response element. (F) EMSA experiment using a control RNA probe.