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T-STAR protein directly regulates Nrxn1-3 AS4 splicing. In the cerebellum T-STAR concentrations are low and most of the Nrxn1-3 mRNA isoforms include AS4 as a result. In the forebrain-derived regions T-STAR concentrations are high, and there are also increased levels of AS4 splicing exclusion resulting from this. Sam68 protein levels are similar across the brain regions.
