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. 2013 Apr 25;7(4):e2184. doi: 10.1371/journal.pntd.0002184

Table 1. Results of the EQA study for laboratories performing ELISA for the serological detection of West Nile virus.

Samples
#12,WNV #5,WNV #7,WNV #6,WNV #1,WNV #3,WNV #8,WNV
BM140375 BM140375 BM140375 BM140375 BM141170 WM67/11 (Greece) #9,DENV #4,JEV #14,TBEV #11,USUV
1∶1 1∶2 1∶4 1∶8 1∶2 1∶2 1∶2 1∶2 1∶2 1∶2 1∶2 #2,Neg #13,Neg
Lab IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG Sc
No. (+) (+) (+) (+) (+) (+) (+) (+) (−) (+) (+) (+) (+) (+) (−) (+) (−) (+) (−) (+) (−) (+) (−) (−) (−) (−) %
32H + +* + + + + +* +* +* + +* * 100
19F + + + + e* + +* + + + + +* +* e* e +* 100
31B + +* + +* + +* + +* + +* + +* + +* +* +* +* +* +* +* +* 100
29bF + +* + +* + +* +* + +* + +* +* +* +* +* +* +* 100
38P + + e +* +* +* +* + + +* +* +* 100
14E + + + + + e* + + + + + e +* * 92
3F + +* + +* + +* +* + +* + +* +* * +* +* +* + 92
40F + + + + e + + + + + + + + + e 92
23F + + + + + + + + + + + + + +* + + + +* 92
7E + + + + + + + + + + + + 85
36bF + + + + + + + + + + + + + e e + 85
16aE + + + + + e + + + + + 85
6H + + + + + + + + + + + + 85
2E e + e + e + e + + + + + + + 85
15E + + e + + + +* 85
45F + + e + + + + + + + + 85
37H + + + + e e + + + + + + e + 77
20P + + e + e e e + + + +* * 77
39F + nd + nd e nd nd + nd + nd nd nd nd nd nd nd nd 77
47E + + + + e e + + + + + 77
8S + + + + + + + + + + + + + + + + + + + 69
13F + + + + + + + + + + + + + + 69
1F + + + + + + + + + + + + + + + + 69
25F + + + + + + + + + + + + + + + + 69
24E + nd + nd e nd nd nd + nd nd nd nd nd nd nd nd 69
43P + nd e nd nd nd e nd + nd nd nd nd nd nd nd nd 69
44H + + + + + + + + + + + + + 69
4F + + + + + + e + + + + + + + + + + + 62
18F + + + + + + + + + + + + + + + + + 62
30F + + + + + + + + + + + + + + + + + + + 62
26aF + + + + e + + + + + + + + + + + + + 62
27P + + + + + + + + + + + + + + 62
12T + + + + + + + + + + + + + + + + + + + + + + + + + + 54
33H nd nd nd nd nd + nd nd nd nd nd nd nd nd 54
46H + + + + 54
Sc % 94 91 71 69 94 100 77 43 74 66 66 63 94

WNV: West Nile virus; DENV: dengue virus; JEV; Japanese encephalitis virus; TBEV: tick-borne encephalitis virus; USUV: Usutu virus; Neg: negative control; Sc: Score; IgM: Immunoglobulin M; IgG: Immunoglobulin G; nd: not done;

*

: reactivity also with heterologous flaviviruses;

S

: commercial ELISA InBios;

H

: ELISA in-house test;

E

: commercial ELISA Euroimmun;

F

commercial ELISA Focus;

B

: commercial ELISA Bioscreen;

P

: commercial ELISA Panbio;

T

: commercial ELISA IDVET.