Skip to main content
. 2013 Apr 25;7(4):e2184. doi: 10.1371/journal.pntd.0002184

Table 2. Results of the EQA study for laboratories performing IFA for the serological detection of West Nile virus.

Samples
#12,WNV #5,WNV #7,WNV #6,WNV #1,WNV #3,WNV #8,WNV
BM140375 BM140375 BM140375 BM140375 BM141170 WM67/11 (Greece) #9,DENV #4,JEV #14,TBEV #11,USUV
1∶1 1∶2 1∶4 1∶8 1∶2 1∶2 1∶2 1∶2 1∶2 1∶2 1∶2 #2,Neg #13,Neg
Lab IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG IgM IgG Sc
No. (+) (+) (+) (+) (+) (+) (+) (+) (−) (+) (+) (+) (+) (+) (−) (+) (−) (+) (−) (+) (−) (+) (−) (+) (−) (+) %
17E 1∶320* 1∶80* 1∶20* 1∶20* 1∶320* 1∶160 1∶160* 1∶40* 1∶2560* * * * 1∶10* 100
21E 1∶40 1∶160 1∶20 1∶80 1∶20* 1∶20* 1∶20 1∶320* 1∶80 1∶160 1∶20 1∶80* +* 1∶10* 1∶10 1∶10* 92
29aH 1∶256* 1∶128* 1∶64* 1∶32* 1∶1024* 1∶128 1∶1024* 1∶64* 1∶2048* * 1∶32* +* 1∶16* 92
35E + + + + + + e* + + + + + 85
34E + + + + * * + + + + + * * * * * 85
16bE + + + + * * + + + + + * * * * * 85
11E + + + + + + + + + +* 85
28H + + + + + + + + + + + 85
9E 1∶100 1∶100* 1∶100 1∶32 1∶320 1∶10 1∶320* 1∶100 1∶3200 1∶10 85
36aS 1∶320 1∶320 1∶160 1∶80 1∶640 1∶160 1∶640 1∶160 1∶2560 1∶80 1∶40 1∶40 69
41H * + + + + + 62
Sc % 91 91 73 55 100 100 91 55 100 91 64 82 100

WNV: West Nile virus; DENV: dengue virus; JEV; Japanese encephalitis virus; TBEV: tick-borne encephalitis virus; USUV: Usutu virus; Neg: negative control; Sc: Score; IgM: Immunoglobulin M; IgG: Immunoglobulin G; nd: not done;

*

: reactivity also with heterologous flaviviruses;

E

: commercial IFA Euroimmun;

H

: IFA in-house test;

S

: commercial IFA Scimedx.