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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Mutat Res. 2012 Dec 19;0:89–96. doi: 10.1016/j.mrfmmm.2012.12.002

Figure 5. DNA topoisomerase I stimulates BLM unwinding activity using RNA20:DNA33hybrid duplex substrates.

Figure 5

The specific activity of BLM on RNA20:DNA33 hybrid duplex substrates in the presence or absence of DNA topoisomerase I was calculated from curves in Fig 4B by plotting the initial velocity of the reaction at 1 minute against protein concentration used. The slope of the line indicates the specific activity and is indicated in the graph (BLM alone: 2.75 fmol unwound/ min/ nM BLM); BLM with DNA topoisomerase I: 3.72 fmol unwound/ min/ nM BLM).