Figure 4. Overexpression of SNF5 disturbs epigenetic regulation and enhances differentiation.

(A–D) Exogenous SNF5 was overexpressed in NCCIT cells and 72 h later, SNF5, OCT4, EZH2, and loading control histone H3 were analyzed by western blot (A). After exogenous SNF5 transfection, glycerol density centrifugation assay was performed. Fractions of 0.5 ml of the 10 ml 10∼30% glycerol gradient were collected and subjected to western bolt analysis for various BAF complex subunits (B). NOMe-seq was performed indicated OCT4 target regions (C and D). The data is representative of three biological experiments. (E) After overexpression, SNF5 binding at the DNA regulatory regions of OCT4 target genes were analyzed by quantitative PCR.