Figure 2.
Effect of warfarin pre-treatment on adenoviral transgene expression. A replication-deficient recombinant adenovirus encoding the Lac-Z gene (6×108 PFU) was injected in control- (Adenovirus) or warfarin-pretreated animals (Warfarin and Adenovirus). Twenty-four hours later, liver biopsies or adrenal glands were collected. A: Measurement of the β-galactosidase protein in the samples was performed. The data presented are percentages of mean +/− SEM from untreated animals (n = 2), adenovirus-injected animals (n = 10) and adenovirus-injected animals, pre-treated with warfarin (n = 10). 100% represents the average of β-galactosidase activity in the adrenal glands (100% = 7584 β-gal units/mg of protein) or liver (100% = 24805 β-gal units/mg of protein) of adenovirus-injected animals. Statistical test: ANOVA (Prism, Graph-Pad softwares). B) Total RNA were collected from individual adrenal glands or liver biopsies, reversed-transcribed and subjected to quantitative PCR to detect LacZ or 18S RNA. The ratio LacZ/18S of liver or adrenal glands collected from animals injected with adenovirus was set at 100%. Data presented are duplicate determinations from a single adrenal gland and liver biopsy and is representative of 4 independent experiments.