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. 2013 Apr 25;8(4):e62919. doi: 10.1371/journal.pone.0062919

Figure 4. Subcellular localization of BODIPY-Cholesterol in the mouse oocyte.

Figure 4

Zona-intact ovulated oocytes were incubated with the fluorescent cholesterol probe for 15 min at 37°C. (A,B) Oocytes continuously incubated with BPY-Chol were imaged at 15 and 50 min. (D,E) Pulse-chase experiment. After incubation, BPY-Chol was washed and followed in time. (C,F) Fluorescence intensity quantified with ImageJ software. The bars represent the mean ± SEM of a total of 15 oocytes for continuous exposition experiment and 15 oocytes for pulse-chase experiment. Comparison of mean values for each subcellular compartment over time was performed using Student t test. Asterisks denote significant differences (P<0.01). Fluorescence of oocytes measured after 90 min was normalized to 100%.