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. 2013 Apr 25;8(4):e62919. doi: 10.1371/journal.pone.0062919

Figure 5. Effect of cholesterol depletion and repletion on oocyte cholesterol content.

Figure 5

Zona-intact ovulated oocytes were pretreated with 15 mM MβCD for 30 min at 37°C to remove cholesterol. Cholesterol repletion was carried out incubating MβCD-treated oocytes with MβCD/Chol complexes. After depletion/repletion treatment, oocytes were washed and incubated with BPY-Chol for 15 min at 37°C. (A) Control, (B) depleted and, (C) depleted/repleted oocytes labeled with BPY-Chol. (D) Fluorescence intensity quantified with ImageJ software. Bars represent the mean ± SEM of 3 independent experiments from a total of 14 control oocytes, 23 depleted oocytes, and 17 depleted/repleted oocytes. Comparison of mean values was performed using Bonferroni test. Different letters (a-b) denote significant differences (P<0.05).