Figure 1. Bioluminescence imaging of bacterial distribution after intranasal inoculation with bioluminescent C.rodentium.

10⁹ colony forming units (cfu) of bioluminescent C. rodentium were administered intranasally to 8 week old CD1 outbred female mice in 20 µl (n = 3), 10 µl (n = 2), 5 µl (n = 3) and 2.5 µl (n = 2) of PBS. Images were acquired using an IVIS spectrum system, and are displayed as images of peak bioluminescence, with variations in colour representing light intensity at a given location. Red represents the most intense light emission, while blue corresponds to the weakest signal. The colour bar indicates relative signal intensity (as photons s⁻¹ cm² sr⁻¹). Two representative mice shown for each group.