Figure 3. The requirement for ADR1-L2 in lsd1 rcd is P-loop dependent.

(A) Four-week-old plants of the indicated genotypes were sprayed with BTH or water. ADR1-L2 and AAA indicate adr1-L2 lsd1 plants expressing C-terminally HA-tagged wild-type ADR1-L2 or the mutated P-loop allele ADR1-L2_AAA, respectively. In both transgenics expression is driven by the native ADR1-L2 promoter. Pictures of plants were taken 5 dpi. (B) Protein from the indicated genotypes was extracted before or after BTH treatment, run on a denaturing gel, and probed with anti-HA antibody. Ponceau-stained blot shows relative loading.