Figure 1. Characterization of growth and pigment production in B. pumilus SF214.

(A) Growth curve of B. pumilus SF214 at 37°C in rich (LB) medium (black symbols) was determined by following over time the optical density of the culture at 600 nm. Pigment production (white symbols) was determined by measuring over time the optical density of 1 ml of cell-free culture supernatant at 410 nm. (B) Growth rate (continuous line) and pigment production (dashed line) of SF214 at different growth temperatures in rich (LB) medium. Pigment production was evaluated by measuring the optical density of 1 ml of cell-free culture supernatant at 410 nm after 24 hours of growth. (C) Absorbance spectrum between 300 and 500 nm of 360 µg of cell extracts of SF214 grown at 25°C in minimal (S7) (black symbol), rich (LB) (gray symbol) and sporulation-inducing (DS) medium.