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. 2013 Apr 25;8(4):e62093. doi: 10.1371/journal.pone.0062093

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© 2013 Manzo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Microscopy analysis of cells from different fields observed by phase contrast, immunofluorescence with anti-TasA primary antibody and Texas Red conjugated secondary antibody and autofluorescence. Merged panels of autofluorescence-immunofluorescence are shown. Panel A reports examples of cells that are not autofluorescent and that are also not recognized by anti-TasA, indicated by arrows. Panel B focuses on examples of autofluorescent cells, with some recognized (yellow in the merge) and some not recognized by anti-TasA antibody.