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. 2013 Apr 25;8(4):e61913. doi: 10.1371/journal.pone.0061913

Figure 1. SDS-PAGE of the purification step by anion exchange chromatography (MonoQ 5/50 GL column).

Figure 1

(A) Protein purification from MC4100 cells cultivated in TGYEP medium supplemented with 2 µM sodium selenite and 2 µM sodium molybdate; 12% (w/v) resolving gel; M: molecular weight marker; L: sample applied to the column; F, column flow-through; 1–5: elution fractions. (B) Protein purification from MC4100 cells cultivated in TGYEP medium supplemented with 100 µM sodium selenite and 100 µM sodium molybdate; 8% (w/v) resolving gel; M: molecular weight marker; L1: sample loaded on the size exclusion column; L2:sample loaded on the anion exchange column; 1–4: elution fractions from the anion-exchange column. Polypeptides labeled I though VIII were those identified by mass spectrometry and are listed in Table 1.