Fig. 3.

Treatment of non-transgenic neurons with Aβ partially increases AcH3 toward levels that are increased by TSA, which reduces Aβ 1–42 toxicity. a The AcH3 signal is significantly higher after Aβ (solid circles) or positive control of TSA treatment (open circles) compared to vehicle-treated neurons (gray circles). n = 98–169 neurons from 2–4 animals for each treatment group. b Increased toxicity from Aβ was partially reversed by TSA in non-transgenic middle-age neurons, but not in old neurons. Indicated neurons were treated with TSA, Aβ 1–42 and/or vehicle in cultures derived from 11 month (open bars) and 19 month (solid bars) mice. Percentage dead was measured as 100 × number dead / (number dead + live). Data represent 1826–3539 neurons per group from two mice. *p < 0.001, **p < 0.0001 by Student’s t test