Fig. 5.

The effect of γ-rays and H₂O₂ on survivin expression is age dependent. a, c Young and old cells were either sham-treated or exposed to γ-rays (30 Gy) or H₂O₂ (0.2 μM) and then were incubated for the indicated periods of time, before being harvested and proteins extracted. Thirty micrograms of proteins was used for immunoblotting analysis using the indicated antibodies. The numbers below the gels indicate the induction/reduction folds in the expression levels after normalization against β-actin. b, d Graphs showing the age-dependent level of Bax/Bcl-2 ratio after treatment with γ-rays or H₂O₂, respectively. Error bars represent standard deviation of at least three different experiments. e Western blot analysis using the indicated antibodies in young TP53 shRNA-expressing cells and their control counterparts treated with γ-rays (30 Gy). The numbers below the gels indicate the fold of induction/reduction in the expression levels as compared to the basal level (time 0)