Table 1.

Functional effects of phosphorylation of kinetochore/centromere localized proteins.

Protein	Modifier	Function/process	Targets
CDC20/Phos:1	Decreased	Ubiquitin protein ligase activity
	Increased	Spindle checkpoint
BUB1B/Phos:2	Increased	Protein binding	BUB1
	Increased	Protein binding	PP2A
	Increased	Protein kinase activity
	Increased	Attachment of spindle microtubules to kinetochores
	Increased	Metaphase plate congression
BUB1B/Phos:3	Increased	Metaphase plate congression
	Increased	Chromosome segregation
	Increased	Spindle checkpoint
	Increased	Protein localization to kinetochore	MAD1L1, MAD2L1
	Decreased	Negative regulation of protein phosphorylation	NDC80
BUB1B/Phos:4	Increased	Attachment of spindle microtubules to kinetochores
	Increased	Inhibition of mitotic anaphase-promoting complex activity
	Increased	Metaphase plate congression
AURKB/Phos:1	Increased	Protein kinase activity
	Increased	Chromosome segregation
	Increased	Metaphase plate congression
	Increased	Spindle checkpoint
ATM/Phos:2	Increased	Protein kinase activity
	Increased	Spindle Checkpoint
HHTA1/Phos:2	Increased	Protein localization to chromosome, centromeric region	SGOL1
H3T3ph	Increased	Protein binding	BIRC5
	Increased	Protein localization to chromosome, centromeric region	AURKB, CDCA8, INCENP, BIRC5

PRO terms retrieved using the search query: “Taxon ID 9606 (human) AND Ontology ID GO:0000776 (kinetochore) AND Modifier NOT NULL OR Taxon ID 9606 (human) and Ontology ID GO:0000779 (condensed chromosome, centromeric region) and Modifier NOT NULL.” Results were restricted to phosphorylated proteins only by selecting “Phosphorylated forms” from the Quick Links menu. Protein binding partners were obtained from the “Interaction with” column of the Functional Annotation section of the PRO entry page. Targets for the annotation terms “Protein localization to the kinetochore,” “Protein localization to the chromosome, centromeric region,” and “Negative regulation of protein phosphorylation” were obtained from the comment column of the PAF.