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. 2010 May 5;30(18):6273–6281. doi: 10.1523/JNEUROSCI.2648-09.2010

Figure 4.

Figure 4.

Stimulation of [35S]GTPγS binding in hippocampal homogenates of HFD-fed (filled circles) and SD-fed (open circles) mice by various concentrations of the cannabinoid receptor agonist HU-210. Assays were performed in the presence of GDP (30 μm) and [35S]GTPγS (0.05 nm) and were incubated for 60 min at 30°C as described in Materials and Methods. Nonspecific binding was determined in the presence of unlabeled GTPγS (30 μm). Basal binding was measured in the absence of receptor agonist and defined as 0% in each experiment. Data are expressed as percentage stimulation above basal [35S]GTPγS binding and are the means ± SEM, n = 2, all performed in quadruplicate. EC50 = 2.29 ± 0.04 nm (SD), 2.45 ± 0.06 nm (HFD); Emax = 68.83 ± 0.90 (SD), 68.74 ± 1.40 (HFD). Unpaired t test analysis, two-tailed: p = 0.9802.