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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Thromb Haemost. 2012 May;10(5):870–880. doi: 10.1111/j.1538-7836.2012.04679.x

Figure 6. RNAR9D-14T inhibits factor V activation by thrombin.

Figure 6

A) Silver stain analysis of FV activation by thrombin in the presence of saturating RNAR9D-14T or RNAmut. FV denotes uncleaved factor V, VaH is the factor Va heavy chain, and VaL is the factor Va light chain. The numbers above the lanes indicate the assay incubation time in minutes (’), and the numbers on the left represent the protein standard sizes. B) Chromogenic assay for FVa cofactor activity. FV (250 ρM) was activated by thrombin (3 nM) in the presence of RNAR9D-14T or RNAmut and time points were diluted into a prothrombinase chromogenic assay where the rate of thrombin generation was dependent upon the concentration of FVa formed. Squares (■) represent no aptamer, triangles (▲) are 500 nM RNAR9D-14T, inverted triangles (▼) are 100 nM RNAR9D-14T, diamonds (◆) are 20 nM RNAR9D-14T, and circles (●) are 500 nM RNAmut. The data were normalized to a parallel control experiment where FV was fully activated in the absence of RNA and are expressed as a percentage of the FVa activity of fully activated FV. The data are single experiments that are representative of three independent experiments.