Figure 4.

Replication of Colombian H5N2 viruses in vitro. MDCK (A) cells were infected at an MOI of 0.01, culture supernatants were collected at 0, 24 and 48 hpi, and virus was titrated as TCID₅₀. (B) NHBE cells were maintained in culture at an air/liquid interface and infected at an MOI of 0.1. At 6, 24, 48 72 and 96 hpi, medium was added to the apical surface. Medium was collected after 30 min, and virus was titrated in triplicate as TCID₅₀. Error bars represent the standard error of the mean. * P<0.05.