FIG. 3.

Neprilysin-deficient islets exposed to palmitate are not protected against reduced glucose-stimulated ATP levels or nitrative stress. ATP levels in response to 2.8 and 20 mmol/L glucose stimulation (A) (n = 5) and nitrate/nitrite levels (B) (n = 7) from C57BL/6 and C57BL/6.NEP^−/− islets after 48-h culture in the absence or presence of 1 mmol/L palmitate. White bars, C57BL/6 islets; black bars, C57BL/6.NEP^−/− islets. Data are means ± SEM. *P < 0.05 vs. control; ‡P < 0.005 vs. 2.8 mmol/L glucose.