FIG. 4.

ATGL-overexpressing hearts exhibit reduced palmitate oxidation after diabetes. Hearts from male 40- to 50-week-old mice were perfused for 60 min ex vivo with 1.2 mmol/L [9, 10-³H]palmitate, 5 mmol/L [U-¹⁴C]glucose, and 50 μU/mL insulin (n = 5–7; *P < 0.05). Heart rate (A), glucose oxidation (B), palmitate oxidation (C), and Krebs cycle acetyl-CoA production (D). β-hydroxyacyl-CoA dehydrogenase enzyme activity (HADH) (E), cardiac Pgc1α mRNA expression (F), palmitoyl CoA (G), and ceramides (H) in 12- to 14-week-old WT and MHC ATGL mice (n = 6–8) in the absence or presence of diabetes. +P < 0.001 vs. WT-SAL; *P < 0.001 vs. WT-SAL; @P < 0.001 vs. WT STZ.