FIG. 7.

PDL does not convert multipotent progenitors to the β-cell lineage. A: Lineage-tracing scheme used to assess the cellular origin of β-cells after PDL. B and C: Tamoxifen-dependent recombination. Images of tail pancreas from mice treated with a single dose of tamoxifen (0.015 g/kg) followed by sham operation or PDL (mice killed at 7 days). B: DAPI (blue), YFP (green), insulin (red), pancytokeratin (white). C: DAPI (blue), YFP (green), insulin (red), amylase (white). D–F: Quantitative analysis of multicellular clones, assessed by the presence of ductal clusters of YFP⁺ pancytokeratin⁺ cells (D), β-cell clusters of YFP⁺ insulin⁺ cells (E), and pancreatic acinar cell clusters of YFP⁺ amylase⁺ cells (F). Scale bars: 50 μm. *P < 0.05, ligated vs. unligated pancreas tail. D, day.