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. 2013 Apr 16;62(5):1519–1526. doi: 10.2337/db12-1066

FIG. 1.

FIG. 1.

Electrical stimulation (ES) induces ATP-dependent glucose uptake in myotubes. Primary myotubes were exposed to tetanic stimulation (400 pulses, 1-ms each at 45 Hz), insulin (100 nmol/L), or exogenous ATP (100 μmol/L). 2-NBDG uptake was assayed as indicated in research design and methods. A: Representative image of 2-NBDG uptake induced by ES, ATP, or insulin, measured 15 min after stimulation. B: 2-NBDG uptake quantification. Myotubes were preincubated for 30 min with or without apyrase (2 units/mL) (C) or suramin (100 μmol/L) (D), and electrically stimulated. Myotubes were preincubated for 30 min with or without apyrase (2 units/mL) (E) or suramin (100 μmol/L) (F) and then treated with exogenous ATP. Values represent the mean ± SD. **P < 0.001 vs. basal group. Data are from at least five independent experiments corresponding to 90 cells analyzed per condition. Scale bar: 10 μm.