FIG. 5.

ATP-dependent glucose uptake and GLUT4myc translocation require PI3Kγ and Gβγ activation. Skeletal myotubes were preincubated with PI3Kγ inhibitor AS6052540 (30 nmol/L) for 30 min or transduced with Adβark-ct (MOI 5000) for 48 h and then stimulated with ATP, as indicated. A: 2-NBDG uptake. B, C, and D, GLUT4myc translocation in myotubes overexpressing wild type (wt), kinase dead (kd), or PI3Kγ-myr. Values represent the mean ± SD. **P < 0.001 vs. basal group, †P < 0.01, and ††P < 0.001 relative to the ATP-stimulated control condition. Data are from at least four independent experiments.