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. 2013 Apr 16;62(5):1519–1526. doi: 10.2337/db12-1066

FIG. 6.

FIG. 6.

ATP signaling toward AS160 and Rab8A in L6-GLUT4myc cells. L6 cells that stably expressed GLUT4myc were transiently transfected with siRNAs, as indicated in research design and methods. A: Rab8A and Rab10 knockdown. L6-GLUT4myc myoblasts were transfected with siRNA to Rab8A, to Rab10, or with nonrelated (NR) siRNA. Extracellular exposure of the myc epitope was detected in nonpermeabilized cells as described in research design and methods. B and C: Quantification of the effect of Rab8A or Rab10 knockdown on ATP-induced GLUT4myc translocation. Values are the mean ± SD. **P < 0.001 vs. basal group, ††P < 0.001 relative to the corresponding siRNA NR condition. Data are representative of at least three independent experiments.