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UCP2 is efficiently deleted specifically from islet α-cells of UCP2AKO mice. A: Schematic diagram of the UCP2-targeting construct. B: Cre and UCP2 tissue expression measured by standard PCR. C: Immunostaining for UCP2 protein (red) and glucagon (green) in dispersed islet cells from Gcg-cre and UCP2AKO mice. UCP2AKO islet cells were also stained for Cre (red) and glucagon (green) or insulin (green). Colocalization is indicated by yellow and arrows. N = 3.
