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. 2013 Apr 16;62(5):1581–1592. doi: 10.2337/db12-0167

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© 2013 by the American Diabetes Association.

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FIG. 3. — Effect of single candidate factors on induction of pancreatic progenitors. A: Single mesenchymal factors tested in combination with R/K/N. Quantification of PDX1 expression in samples treated with single mesenchymal factors compared with control. Control condition is set to “1.” Ten independent fields were used for counting. Statistical analysis was performed using the Student t test. *P < 0.05 and **P < 0.01. B: Percentage of proliferating cells in control and experimental samples treated with single mesenchymal factors. Total number of cells counted for each condition is ≥14,000. C: Percentage of proliferating pancreatic progenitor cells in control and experimental samples treated with single mesenchymal factors. Total number of cells counted for each condition is ≥14,000. D: Immunostaining with PDX1 and pH3 indicates induction of progenitor cells under control, TGF-β2, and TGF-β3 conditions. D: Scale bar = 200 μm in all images.