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. 2013 Apr;61(4):313–324. doi: 10.1369/0022155413477115

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© The Author(s) 2013

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Figure 1. — mRNA expression of nephrin in developing pronephric glomerulus. (A1–4) Zebrafish. Nephrin is expressed in the medial half of the vesicular nephron primordia as early as 34 hours postfertilization (hpf) (A1). A pair of nephrin-expressing regions is fused at the midline to form a single glomerulus by 2 days postfertilization (dpf) (A2), and its expression is preserved within the glomerulus in 3 dpf (A3) and 4 dpf (A4) embryos. (B1–4) Medaka. Nephrin-expressing regions remain separated throughout their development. Nephrin is already expressed at the most medial portion of the vesicular nephron primordia at 3 dpf (B1). Nephrin expression is found as a C-shaped epithelium at 4 dpf (B2) but has a concave and convex appearance at 5 dpf (B3) and 6 dpf (B4). Bar scale, 50 µm.