Figure 1.

A representative confocal microscope image of connexin 43 expression in the murine subventricular zone (SVZ) (coronal view). BrdU (green) marks a subpopulation of quiescent astrocytic cells that are presumed to be neural stem cells (NSCs) (Shen et al. 2008; Tavazoie et al. 2008; Kazanis et al. 2010). The algorithm described herein allows detection and assignment of connexin expression to BrdU⁺ NSCs (arrows) by performing a nuclear neighborhood search at a pixel-level resolution. Bar = 10 µm.