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Functional characterization and determination of binding specificity of wild-type (wt) and mutant CD301 (mut) determined by glycan ELISA with 16 immobilized glycoconjugates. The composition of glycans conjugated to polyacrylamide (PAA) is given by the pictograms. Measurements were performed in triplicate; error bars indicate the standard error of the mean. PAA served as background control.
