Figure 2.

sTSLIM CAD diagram with light path shown. A blue (bl) or green (gl) laser travels through an acoustic optic modulator (aom) and is expanded using a beam expander (bx). Scanning of the beam is provided by the galvo mirror (g) and the specimen (s) moves through the scanned light-sheet in the X- and Z-directions via micropositioners (mp) to produce well-focused specimens across their width and a Z-stack of images, respectively, which are recorded through an objective of an Olympus microscope (mo) and a QImaging Retiga digital camera (not shown).