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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: Biochemistry. 2013 Mar 18;52(13):2337–2347. doi: 10.1021/bi400175d

Figure 6. ANF/ATP-dependent ANF-RGC activity in the membranes of kidney (A) and adrenal gland (B) of the wild type and 669WTAPELL675 genetically modified mice and of COS cells transfected with wild type ANF-RGC or its 669WTAPELL675 deletion mutant (C).

Figure 6

Membrane fractions of the kidneys and the adrenal glands were isolated from the wild type [669WTAPELL675(+/+)], the heterozygous [669WTAPELL675(+/−)], the homozygous [669WTAPELL675(−/−)] mice and COS cells expressing wild type ANF-RGC or its 669WTAPELL675 deletion mutant. These were evaluated for guanylate cyclase activity in the presence of indicated concentrations of ANF and 0.8 mM ATP and cyclic GMP formed was measured by radioimmunoassay. The experiment was done in triplicate and repeated three times with separate membrane preparations. The results presented (mean ± SD) are from these experiments.