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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: Biochemistry. 2013 Mar 18;52(13):2337–2347. doi: 10.1021/bi400175d

Table 1.

Guanylate cyclase activity in the membrane fraction of the kidney and adrenal gland of the wild type and ANF-RGC 669WTAPELL675 modified mice.

Tissue/ activity Wild type 669WTAPELL675(+/−) 669WTAPELL675(−/−)
Kidney 42 ± 4 40 ± 6 41 ± 5
Adrenal gland 78 ± 9 75 ± 10 81 ± 9
COS cells 23 ± 3 22 ± 3

The membrane fractions were prepared as described in the Experimental Procedures section. Membranes of the COS cells expressing wild type ANF-RGC and its 669WTAPELL675 deletion mutant were assayed as control. The experiment was done in triplicate and repeated three times with different membrane preparations. The results are mean ± SD from these experiments. The activity is expressed as pmol cyclic GMP min−1 (mg protein)−1.