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. 2012 Jun 6;32(23):7970–7985. doi: 10.1523/JNEUROSCI.0154-12.2012

Figure 1.

Figure 1.

Functional characterization and morphological visualization of mitral and tufted cells. A, The sequence of optical imaging, juxtacellular recording, and labeling methods for the visualization of a functionally identified neuron. B, Optical imaging of intrinsic signals showing TMT-responsive glomeruli (dark spots) in the exposed dorsal surface (white line) of the left OB. A recording micropipette was lowered near the glomerulus (arrowhead). A, Anterior; L, lateral. C, Juxtacellular single-cell recording from a TMT-responsive MC. Spike responses to TMT odor are shown with raster representation (top traces) and a peristimulus time histogram (bottom graph). D, Juxtacellular single-cell electroporation. Current-induced firing of the neuron (top trace) is perfectly locked to the positive current pulses (400 ms on/off; bottom trace) applied to the recorded neuron, indicating successful electroporation. E, Visualized cell body and dendrites of the labeled MC in the OB, a dense axon collateral bush in the pars externa of the anterior olfactory nucleus (AONpE), an axon collateral bush in the anterior piriform cortex (APC), and axon collaterals in the tenia tecta (TT), posterior piriform cortex (PPC), and lateral entorhinal cortex (LEC). Axons were clearly visualized in all OC regions. F, Three-dimensional reconstruction of a single TMT-responsive MC. The left hemisphere of the brain containing the labeled cell is shown for frontal (left), lateral (top right), and ventral (bottom right) views. Axons, primary dendrites, and lateral dendrites are indicated by yellow, green, and magenta, respectively. The mitral cell layer (MCL) of the OB and different areas of the OC are indicated by distinct colors and superimposed on the line tracing of the brain surface (brown). Abbreviations: AONpP, Pars principalis of anterior olfactory nucleus; OT, olfactory tubercle; OTCAP, cap part of olfactory tubercle; OTCO, cortical part of olfactory tubercle; nLOT, nucleus of the lateral olfactory tract; ACO, anterior cortical amygdaloid nucleus; PLCO, posterolateral cortical amygdaloid nucleus; A, anterior; D, dorsal; L, lateral.