Fig. 5.

Intestinal expression of DeltaD in WT and ascl1a−/− embryos. (A) Both mutant and WT 5 dpf intestines and brains were dissected for western blot. Brains for both WT and ascl1a−/− demonstrate similar levels of DeltaD and the loading control β-actin. ascl1a−/− intestines lack DeltaD while there are similar levels of β-actin compared to WT. DeltaD is approximately 100 kD while β-actin is 55 kD. (B) Total protein as visualized by induced fluorescence on stain-free gel. Proteins are visualized after electrophoresis but before transfer to membrane as an alternative loading control to β-actin. The protein gel for DeltaD was run without β-mecaptoethanol (BME) and shown to the left while protein gel for β-actin was run with β-mecaptoethanol (BME) and is shown to the right. The same volume of protein from the same sample was loaded on both the BME and no BME gels.